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Open Journal of Neuroscience

ISSN: 2075-9088
Volume 5, 2017

Open Journal of Neuroscience, 2014, 4-1 [Research Article]

N-Methyl-D-Aspartate Receptor Activation Interacts with Electrical High Frequency Stimulation in the Rat Caudate Nucleus in vitro and in vivo

Ramya Varatharajana,b,*, Kevin Josepha,b, Susanne Loefflera,d, Henriette Fuellgrafa, Ulrich G. Hofmannc,e, Andreas Mosera,f

a Neurochemical Research Group, Department of Neurology, University of Lübeck,  Germany
b Graduate School for Computing in Medicine and Life Science, University of Lübeck, Germany
c Biosignal Processing and Neuro-Engineering, Institute for Signal Processing, University of Lübeck, Germany
d Department of Neuroscience, Karolinska Institute, SE-17177 Stockholm, Sweden
e Neuroelectronic Systems, Department for Neurosurgery, University Medical Center Freiburg, D-79106 Freiburg, Germany
f Freiburg Institute of Advanced Studies, University of Freiburg, D-79106 Freiburg, Germany

Corresponding Author & Address:

Ramya Varatharajan*
Neurochemical Research Group, Department of Neurology, University of Lübeck,  Germany; Phone: +494515002486; Fax: +494515002936; Email: ramya.varatharajan@neuro.uni-luebeck.de

Article History:
Published: 12th March, 2014   Accepted: 12th March, 2014
Received: 30th January, 2014      

© Varatharajan et al; licensee Ross Science Publishers

ROSS Open Access articles will be distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work will always be cited properly.

Keywords: electrical high frequency stimulation, γ-aminobutyric acid, NMDA receptor, microdialysis, freely moving rats.


Electrical high frequency stimulation is used as treatment for motoric- as well as psychomotoric disorders in various regions of the brain. The biochemical mechanisms of the action of high frequency stimulation underlying the effective results are unclear. Previous studies have demonstrated that electrical 130 Hz stimulation was capable of increasing local γ-aminobutyric acid outflow in the rat caudate nucleus when neuronal pre-activation was present. In the present study, the local effect of electrical high frequency stimulation on γ-aminobutyric was reviewed under conditions of N-methyl-D-aspartate receptor activation. Using in vitro superfusion experiments with tissue slices from the rat caudate nucleus, and co-localized in vivo microdialysis and electrical 130 Hz stimulation of the caudate nucleus in awake and freely moving rats, we can conclude that N-methyl-D-aspartate receptor activation was able to modulate γ-aminobutyric acid outflow induced by electrical high frequency stimulation.

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