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Open Journal of Hematology

ISSN: 2075-907X
Volume 8, 2017



Indexed in:
EMBASE

Open Journal of Hematology, 2015, 6-7 [Research Article]

Reactive oxygen species in hematopoietic stem cells affect culture outcomes under inflammatory conditions

Takashi Ishida1,2, Satoshi Yamazaki2, Hiromitsu Nakauchi2, Masaaki Higashihara1, Makoto Otsu3,*

1 Department of Hematology, Kitasato University Graduate School of Medical Science, Kanagawa, Japan
2 Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, Tokyo, Japan
3 Division of Stem Cell Processing and Stem Cell Bank, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, Tokyo, Japan


Corresponding Author & Address:

Makoto Otsu*
Division of Stem Cell Processing and Stem Cell Bank, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639 Tokyo, Japan; Tel: +81-3-6409-2342; Fax: +81-3-6409-2343; Email: motsu@ims.u-tokyo.ac.jp

Article History:
Published: 7th September, 2015   Accepted: 7th September, 2015
Received: 23rd June, 2015      

© Otsu et al.; licensee Ross Science Publishers

ROSS Open Access articles will be distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work will always be cited properly.

Abstract:

Functions of hematopoietic stem cells (HSCs) are tightly regulated to ensure lifelong normal hematopoiesis within the bone marrow (BM) niche. While HSCs’ status is well orchestrated to uphold their stemness, the fate of HSCs can be altered in response to inflammatory signals caused by environmental changes. The influence on HSCs of inflammatory changes within BM has been explored; however, how each inflammatory cytokine affects the maintenance or differentiation of HSCs remains to be fully elucidated. Considering recent studies demonstrating that reactive oxygen species (ROS) influence HSCs’ fates, we conducted a comprehensive analysis of the effects of seven major inflammatory cytokines on proliferation, differentiation, and ROS level regulation in HSCs / hematopoietic stem/progenitor cells (HSPCs). Our study indicates that inflammatory cytokines in general push HSCs/HSPCs into differentiation, but vary significantly with respect to retention of primitive cells. With definition by cellular immunophenotypes, TNF-α, IFN-γ, and type I IFNs stimulated loss of HSCs, whereas TGF-β and IL-6, and possibly IL-1β as well, showed potential to retain them. Of note is that unaltered ROS levels were compatible with HSC retention, while drastic change, either increase or decrease, in ROS levels presaged HSC loss. Detailed evaluation of links between inflammatory cytokines and ROS regulation appears likely to prove important in understanding HSC biology and translating basic science into clinical applications.



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