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Open Journal of Hematology

ISSN: 2075-907X
Volume 8, 2017



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Open Journal of Hematology, 2015, 6-5 [Research Article]

THE ROLE OF COMPLETE BLOOD COUNT IN THE DIAGNOSIS OF HEMOGLOBIN E IN PSEUDO HIGH LEVEL OF HEMOGLOBIN A2

Alida Harahap1,2,*, Dewi Megawati1,3, Ita M. Nainggolan1,4, Maria Swastika1, Iswari Setianingsih1

1 Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta 10430, Indonesia.
2 Department of Clinical Pathology, Faculty of Medicine, University of Indonesia-Cipto Mangunkusumo Hospital, Jl. Diponegoro 71, Jakarta 10430, Indonesia.
3 Faculty of Medicine and Health Sciences, Warmadewa University, Jl. Terompong  24. Denpasar Bali 80235, Indonesia
4 Ministry of Research Technology and Higher Education, Jl. MH Thamrin 8. Jakarta 10430, Indonesia

Corresponding Author & Address:

Alida Harahap*
Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, 10430, Jakarta, Indonesia; Phone: +62-21-3148695; Fax: +62-21-3147982; E-mail: alida@eijkman.go.id

Article History:
Published: 16th May, 2015   Accepted: 16th May, 2015
Received: 27th March, 2015      

© Harahap et al.; licensee Ross Science Publishers

ROSS Open Access articles will be distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work will always be cited properly.

Keywords: hemoglobin E, hemoglobin A2, hemoglobin concentration, MCV, MCH

Abstract:

Aim: To evaluate the usefulness of complete blood count (hemoglobin concentration, MCV: mean corpuscular volume, and MCH: mean corpuscular hemoglobin), in diagnostic of hemoglobin E (HbE) when hemoglobin analysis showed pseudo high level of HbA2.

Methods: Secondary data were obtained from laboratory records of patients who were referred to the GenNeka Genetic Clinic, Eijkman Institute for Molecular Biology, Jakarta, for thalassemia screening. The patients employed were heterozygous HbE, compound heterozygous HbE with other β-thalassemia mutations, homozygous HbE, and co-inheritance with α-thalassemia, that was confirmed by molecular analysis. Complete blood count (CBC) was examined using an automated blood cell analyzer. HbA2 level was measured by HPLC. Molecular analysis was performed by PCR-RFLP method. Hemoglobin concentration, MCV, and MCH values will be presented as a mean or median, and will be compared between heterozygous HbE and compound heterozygous HbE. A comparison was also done between males and females in the heterozygous HbE group. Homozygous HbE and co-inheritance with α-thalassemia will be described as an extrapolation.

Results: The hemoglobin concentration, MCV, and MCH values were significantly different between heterozygous HbE and compound heterozygous HbE. Only the hemoglobin concentration and the MCV values were significantly different between males and females in the heterozygous HbE group.

Conclusion: CBC is useful in assisting the differentiation of HbE types in misleadingly high HbA2 levels. The sex of the patient has to be considered in the interpretation of CBC results.



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