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Open Journal of Hematology

ISSN: 2075-907X
Volume 8, 2017



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Open Journal of Hematology, 2014, 5-4 [Research Article]

Effect of the Montivipera bornmuelleri snake venom on human blood: coagulation disorders and hemolytic activities

Claudine Accary1,£, Mohamad Rima1,£, Achraf Kouzayha1,2, Walid Hleihel3, Riyad Sadek4, Jean Claude Desfontis5, Ziad Fajloun1,2,**, Souad Hraoui-Bloquet6,*

1 Laboratory of Applied Biotechnology, Azm Center for the Research in Biotechnology and its Application, Doctoral School for Sciences and Technology, Tripoli, Lebanon
2 Faculty of Sciences, Biochemistry Department , Section III, Lebanese University, Tripoli, Lebanon
3 Faculty of Medical Science, USEK, Kaslik-Jounieh, Lebanon
4 American University, Biology Department, Beirut, Lebanon
5 LUNAM, Oniris, Unit of Animal Pathophysiology and Functional Pharmacology, Nantes, France
6 Faculty of Sciences II (Fanar), Lebanese University, B.P. 90656 Jdeidet El Maten, Lebanon

£ Both authors contributed equally to this work

Corresponding Author & Address:

Souad Hraoui-Bloquet*
Faculty of Sciences II (Fanar), Lebanese University, B.P. 90656 Jdeidet El Maten, Lebanon; Phone: +961 1 68 69 83; Email: sdbloquet@yahoo.com

Ziad Fajloun**
Laboratory of Applied Biotechnology, Azm Center for the Research in Biotechnology and its Applications, Doctoral School for Sciences and Technology, Lebanese University, El Mittein Street, Tripoli, Lebanon; Phone: + 961 6 213 255, Fax: + 961 6 213 383, Email: zfajloun@ul.edu.lb

Article History:
Published: 9th June, 2014   Accepted: 9th June, 2014
Received: 16th February, 2014      

© Hraoui-Bloquet and Fajloun et al.; licensee Ross Science Publishers

ROSS Open Access articles will be distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work will always be cited properly.

Keywords: M. bornmuelleri, blood clotting, cytotoxicity, hemolysis, phospholipases A2

Abstract:

Viper’s venom is a source of biopharmaceutical compounds, hence the need to assess the effect of this animal extract on human blood. Here, we studied the blood coagulation disorders and hemolytic activities of the venom of M. bornmulleri viper. The pro-coagulant and anticoagulant effects are analyzed with venom concentrations ranging from 0.4 to 0.0031 mg/mL. Thus, the PT is way above the normal value indicating an anticoagulant activity whereas for the aPTT, the high concentration of the venom showed an anticoagulant activity, but a pro-coagulant effect was occurred when the venom concentration decreases to 0.05 and/or 0.025 mg/mL. Hemolytic tests, performed in suspension (30% RBCs) and on blood agar plate (5% RBCs), show that an increased concentration of the venom going until 1.6 mg cannot produce a hemolytic effect, even in the presence of Ca2+ (hemolysis < 0.5%). Also, on the blood agar plate no hemolytic area appeared even with 0.04 mg of the lyophilized venom. Otherwise, the venom was able to induce a low hemolytic activity (hemolysis = 1.3 %) by acting on L-α-PC used as substrate. In this case, the destruction of erythrocytes increased proportionally to the added amount of phospholipids which are hydrolyzed to fatty acids and lysophospholipids (two toxic substances for RBCs), probably due to the presence of PLA2 in the venom and which are known by their ability to hydrolyze lecithin



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